Laser flow cytometry and cancer chemotherapy: detection of intracellular anthracyclines by flow cytometry.

Evaluation of DNA-content of Peritoneal Lavage Cells by Flow Cytometry in Patients with Gasteric Cancer

Background & Aims: Gastric cancer is the second common cancer in Iran and it has unfortunately no diagnostic signs in early stages. Considering the role of genetic and environmental factors in the development of gastric cancer, this study aimed at evaluating DNA-content of stomach cells in patients with gastric cancer in order to find a reliable index for prediction of disease prognosis and pro...

Detection of bacterial aggregation by flow cytometry

Since the advent of recombinant DNA technology, bacteria have been used often to express foreign proteins [1]. Soluble proteins expressed by bacteria can be expressed either in the cytoplasm or they are secreted to the periplasm or outside the cell. In general proteins expressed in the cytoplasm are expressed to higher levels than if they are secreted[1]. Many hydrophobic and highly expressed p...

Detection of intracytoplasmic cytokines by flow cytometry.

Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years t...

Module : Antibody detection / identification – by Flow Cytometry

Flow Cytometry Analysis of Intracellular Protein

The demand for determining protein levels in a continuously decreasing amount of input cells is currently being developed by scientists and medical doctors. Another demanding area is the splitting of active and inactive (phosphorylated and unphosphorylated, respectively) forms of the protein being monitored, e.g., during different phases of the cell cycle, differentiation or carcinogenesis. The...